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target="_blank">大鼠低氧誘導(dǎo)因子1αelisa試劑盒</a>主要是用于測定兔血清、血漿、組織及相關(guān)液體樣本中的含量或活性。例如適合檢測包括血清、血漿、尿液、胸腹水、灌洗液、腦脊液、細(xì)胞培養(yǎng)上清、組織勻漿等標(biāo)本。樣本是由兩個不同的ELISA檢測抗-HCA表現(xiàn)，其他概率是由NAT或RIBA屢次反響數(shù)據(jù)的證明。<div>　　溫馨提示：使用前，請*閱讀說明書。測定試劑盒是基于經(jīng)典酶聯(lián)夾心技術(shù)原理，只能用于研究用途，不得用于醫(yī)學(xué)診斷。</div><div>　　</div><div>　　實驗原理：</div><div>　　本試劑盒應(yīng)用雙抗體夾心法測定標(biāo)本中大鼠低氧誘導(dǎo)因子-1α（HIF-1α）水平。用純化的大鼠低氧誘導(dǎo)因子-1α（HIF-1α）抗體包被微孔板，制成固相抗體，往包被單抗的微孔中依次加入低氧誘導(dǎo)因子-1α（HIF-1α），再與HRP標(biāo)記的低氧誘導(dǎo)因子-1α（HIF-1α）抗體結(jié)合，形成抗體-抗原-酶標(biāo)抗體復(fù)合物，經(jīng)過*洗滌后加底物TMB顯色。TMB在HRP酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的低氧誘導(dǎo)因子-1α（HIF-1α）呈正相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度（OD值），通過標(biāo)準(zhǔn)曲線計算樣品中大鼠低氧誘導(dǎo)因子-1α（HIF-1α）濃度。</div><div>　　</div><div>　　大鼠低氧誘導(dǎo)因子1αelisa試劑盒保存溫度：</div><div>　　1、常溫保存：通常用于化學(xué)性質(zhì)比較穩(wěn)定的產(chǎn)品，主張保存于枯燥陰涼的地方。</div><div>　　2、4度冷藏：用于常溫下不是很穩(wěn)定的物質(zhì)，保存于冰箱冷藏室。</div><div>　　3、20度冷凍：用于化學(xué)性質(zhì)不穩(wěn)定，常溫下簡單分解的物質(zhì)。</div><div>　　4、80度保存：-些具有生物活性的物質(zhì)，需求保存于特定的-80度的冰箱。</div><div>　　</div><div>　　大鼠低氧誘導(dǎo)因子1αelisa試劑盒性能優(yōu)勢：</div><div>　　1、特異性：</div><div>　　來自抗體或抗原的挑選性?？乖贵w的結(jié)合實質(zhì)上只發(fā)生在抗原的抗原決定簇與抗體的抗原結(jié)合位點之間。因為兩者在化學(xué)結(jié)構(gòu)和空間構(gòu)型上呈互補聯(lián)系，所以抗原抗體反響具有高度的特異性。</div><div>　　2、活絡(luò)性：</div><div>　　該測定法的活絡(luò)度來自作為陳述的酶。酶是一種有機(jī)催化劑，很少量的酶即可誘導(dǎo)很多的催化反響，發(fā)生可供調(diào)查的顯色反響現(xiàn)象，因而該體系常被稱為酶放大體系。</div><div>　　ELISA完成了在細(xì)胞或亞細(xì)胞水平上示蹤抗原或抗體的地點部位，或在微克、乃至納克水平上對其進(jìn)行定量。</div> 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