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</div><div>　　ELISA是一種基于抗原-抗體反應的檢測技術，其中酶標記的二級抗體或親和素與分子之間形成復合物，通過底物酶反應生成熒光或吸光度信號。兔血清總補體ELISA試劑盒利用雙抗原夾心法檢測血清樣品中總補體含量。具體來說，首先將血清樣品加入含有植物血凝素的96孔板孔中固定；然后加入生物素標記的補體C3抗體，與未被固定的補體C3結合；最后加入辣根過氧化物酶標記的蛋白A，與生物素結合的抗體形成復合體。洗滌后加入底物TMB，產生藍色反應產物，加入停止劑停止反應，測定OD值，OD值與樣品中總補體含量呈正相關關系。<br /> </div><div>　　二、操作步驟<br /> </div><div>　　1、取出所需試劑和材料，標記試板。<br /> </div><div>　　2、加入樣品和對照物質。<br /> </div><div>　　3、加入生物素補體C3抗體(第一抗體)。<br /> </div><div>　　4、洗滌板子去除所有未結合的第一抗體。<br /> </div><div>　　5、加入辣根過氧化物酶標記的蛋白A(第二抗體)。<br /> </div><div>　　6、洗滌板子去除所有未結合的第二抗體。<br /> </div><div>　　7、加入底物TMB。<br /> </div><div>　　8、停止反應，并測定吸光度值。<br /> </div><div>　　三、兔血清總補體ELISA試劑盒具有以下優(yōu)點：<br /> </div><div>　　1、靈敏度高，可以檢測到極低濃度的總補體。<br /> </div><div>　　2、準確性高，結果穩(wěn)定可靠。<br /> </div><div>　　3、操作簡單，易于實驗室進行。<br /> </div><div>　　四、應用領域<br /> </div><div>　　兔血清總補體ELISA試劑盒廣泛應用于臨床醫(yī)學、生命科學和免疫學等領域。其中，最常見的應用是檢測患者血清中總補體含量，以診斷某些自身免疫性疾病，如系統(tǒng)性紅斑狼瘡、類風濕性關節(jié)炎和其他結締組織病等。此外，該試劑盒還可以用于研究小鼠模型中的免疫反應，評估藥物對免疫系統(tǒng)的影響等方面。</div><div style="text-align: center;"> <img 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