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href="http:///Products-37030636.html" target="_blank">大鼠低氧誘導因子1αelisa試劑盒</a>標準品和樣品中的目標蛋白與固定化的抗體結合，移除未結合的底物后，加入目標蛋白特異性的生物素化的檢測抗體，經(jīng)過洗滌，向反應孔中加入的鏈霉親和素-HRP偶聯(lián)物。洗滌移除所有未結合的鏈霉親和素-HRP試劑，加入HRP底物溶液，產(chǎn)生有色產(chǎn)物，顏色的深淺與目標蛋白的的含量成正比。<br /> </div><div>　　在檢測大鼠低氧誘導因子1αelisa試劑盒時，需要you質(zhì)的試劑，良好狀態(tài)的儀器，排除各種因素的干擾，正確操作是保證檢測結果準確可靠的必要條件。<br /> </div><div>　　1、加樣后及時放人孵箱，標本較多時，要分批操作。按說明步驟嚴格控制操作時間，防止孵育時間人為延長，導致非特異性結合緊附于反應孔周圍，難以清洗。<br /> </div><div>　　2、顯色劑盡量在臨用前配制，堅持不用過期顯色劑，肉眼可見淺藍色的TMB顯色劑不用。<br /> </div><div>　　3、加酶試劑后用吸水紙在酶標板表面輕拭吸干。<br /> </div><div>　　4、合理安排檢測量，以免反應板過多造成洗板等待時間長。<br /> </div><div>　　性能優(yōu)勢：<br /> </div><div>　　1、包被：采用國內(nèi)精度的包被機，板內(nèi)誤差和板間誤差在5%之內(nèi)；<br /> </div><div>　　2、樣本稀釋液：我公司提供的樣本稀釋液可有效消除血清中某些成份的影響，準確度更高。<br /> </div><div>　　3、其特異性來自抗體或抗原的選擇性，抗原抗體的結合實質(zhì)上只發(fā)生在抗原的抗原決定簇與抗體的抗原結合位點之間，由于兩者在化學結構和空間構型上呈互補關系，所以抗原抗體反應具有高度的特異性。</div> </ul> <div class="hzfpzidjfkus" id="news_sx"> <li id="hzfpzidjfkus" class="sx_left">上一篇：<a 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